병원성 바이러스 중화항체 치료제 개발지원센터

○ Excitation Laser Module: 488nm blue laser (30mW), 638nm red laser (30mW)

- Auto Power Control (APC) accuracy: ±2% for laser diodes ±3% for diode pumped solid-state (DPSS) laser

○ Detection Module

- Channels: 1x forward scatter(FSC), 1x back scatter(BSC), 6x fluorescence(FL)

- Analog-to-digital converters (ADC): 8-channel 20-bit effective resolution

- Light detectors: Photodiode (FSC), Photomultiplier tubes (BSC, FL)

- Fluorescence detection wavelength: 425 nm to 815 nm

- User exchangeable fluorescence optical filters

- Pulse measurement : Height, width, and area for all channels

- All measurements can be correlated against time parameter for kinetic analysis

- Threshold parameter: Single pulse threshold setting, selectable from all channels

○ Sorting chip

- The sorting chip automatically provides the system with nozzle size and optimal setting for stable reliable performance

- Replaceable sorting chip: The sorting chip, including the nozzle, can be easily replaced

- Nozzle size : 70μm, 100μm, 130μm

○ Sample Injection Chamber

- Sample loader: Single, auto-loading tube

- Sample input sizes: 1.5ml, 5.0ml and 15ml tubes or more

- Agitation unit: Eccentric rotation magnetic drive 300rpm speed

- Sample flow pressure: 50kPa to 300kPa (7 to 43 psi)

- Sample flow rate : 6μl/min (minimum)~168ul/min (maximum)

- Fluidics cleaning modes included in software: Automated startup, shut-down, Clean flow cell, Clean sample injection chamber

- Replaceable Sample Path: The sample path, including the sample line and the sample probe, can be easily exchanged

○ Cell Sorting Setup and Performance

- Two-way sorting into 5.0ml and 15.0ml tubes

- Plate sorting deposition : 6, 24, 48 and 96 well plates

- Sorting rate : Using the 70μm sorting chip at 50 kHz, an average threshold of 12,000 events per second can be achieved with >98% purity and >80% yield. A threshold rate up to 30,000 events per second can be achieved without affecting purity but with a decrease in yield based on Poisson’s statistics

- Sorting purity : More than 98%

- Sorting recovery : More than 80%

- Sorting viability : More than 90%

- Index sorting: Post sort analysis of multi-well plate data or equiv, Identify the sorted cells of each well on plots, statistics views and results file or equiv

- Automated calibration setup: optical axis adjustment, droplet calibration, side stream calibration, drop delay calibration

- Automated sort monitoring: software monitors and actively makes adjustments to the drop drive and side stream. It ensures consistently good sort performance and allows detection of clogs, empty tubes

○ Signal processing and system performance

- Fluorescence sensitivity: FITC ≤120 MESF, PE ≤1100 MESF

- Maximum event rate: 100,000 eps

- Fluorescence resolution: <3.0% coefficient of variation (CV): PI stained CEN

- Linearity : FITC R2 > 0.98, PE R2 > 0.98

- Automatic fluorescence compensation

- Analyzer mode: flow cytometry analysis can be performed without droplet and side stream calibration setup